Recorded Webinar (April 2019) | Bcl2Fastq is a Linux-based software that converts base call files generated from an Illumina sequencing run to FASTQ files as well as demultiplex samples. This webinar focuses on Bcl2fastq v2+ and is targeted for new to intermediate users who are interested in the configurable options that this command line program allows. We will cover the following topics: requirements to run Bcl2Fastq, command line basics, commonly used options, frequently asked ‘How To….’ questions.
This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
Learn about the benefits of instrument performance monitoring through Illumina Proactive, a service in which instrument performance data are sent to Illumina to enable proactive maintenance.
This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.
This course covers best practices for your HiSeq 1500/2500. Topics covered include: instrument mode changes and washes, best practices for performing a HiSeq sequencing run, BaseSpace Sequence Hub and data compression options for your HiSeq runs, fluidics and flow cell plate care, and how to prepare the instrument for idling or shutdown.
This course describes the Rapid Run Mode available for HiSeq 2500 or HiSeq 1500. It identifies what is needed to perform a Rapid Run, describes the options for clustering the 2-lane flow cell, and lists ways to reduce data storage requirements for your HiSeq runs.
This video discusses how to choose the appropriate sequencing primers for your run. Since commonly used Illumina indexes, TruSeq™ and Nextera™, utilize different index primer site sequences, it's important that the correct primer is loaded into the HiSeq for sequencing.
This video offers tips and troubleshooting advice related to the HiSeq Instrument computer, including navigating the instrument's Port Manager, formatting hard drives, and setting up a second run.
This video offers tips on the mechanical and thermal systems of the HiSeq, as well as best practices on maintaining your instrument.
This video introduces the fluidics system. Topics include fluidics leaks, syringe pump function, and priming the HiSeq 2500 template loading station.
This video continues to share tips and tricks for the fluidics system started in Part 4 of the series. The video focuses on how to identify and remove blockages from the fluidics lines to ensure the HiSeq instrument is used at its highest capacity.
An Illumina Field Applications Scientist describes what it means to sequence through the insert of your prepared library. We look at Bioanalyzer traces to assess the range of insert sizes in a library.
This video shows you how to use Sequencing Analysis Viewer (SAV) plots to determine if your sequencing insert is too short. We also explore how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.
This video provides an overview of overclustering and how it can impact your sequencing data. An Illumina Field Applications Scientist shows you how to use Sequencing Analysis Viewer (SAV) to look for common symptoms of an overclustered HiSeq or MiSeq flow cell.