NextSeq 500 Training

  • Introductory Topics

    DATE/LENGTH


  • Sequencing Analysis Viewer (SAV): A Beginner's Guide

    Recorded Webinar (December 2020) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.


  • Sequencing: Introduction to Sequencing By Synthesis (SBS)

    Recorded Webinar (January 2021) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.


  • Library Prep

    DATE/LENGTH


  • AmpliSeq for Illumina: Library Prep Protocol

    This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

    30 min


  • AmpliSeq for Illumina: Overview

    This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

    15 min


  • Illumina DNA Prep Library Preparation

    By the end of this course, you will be able to identify the Illumina DNA Prep kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.

    20 min


  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min


  • Instruments & Software

    DATE/LENGTH


  • Diagnosing Suboptimal Clustering in Nonpatterned Flow Cells

    Learn which run metrics and thumbnail images to monitor during a run to diagnose over clustering of a nonpatterned flow cell.

    8 min


  • IT Requirements for Implementing Illumina Proactive

    This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.

    4 min


  • Local Run Manager v2

    This course shows you how to get started using the Local Run Manager v2 software to set up your sequencing runs and view analysis results. Local Run Manager v2 is available for MiniSeq instruments using MiniSeq Control Software v2 or later, NextSeq 500/550 instruments using NextSeq Control Software v4 and later, and MiSeq instruments using MiSeq Control Software v3.

    Languages: French, Italian, German, SpanishChinese (Simplified)

    20 min


  • Optimal Cluster Density Best Practices

    This video discusses optimal cluster density and how over and under-clustering can affect your sequencing data. It also examines common clustering issues and ways to prevent them.

    10 min


  • Data Analysis

    DATE/LENGTH


  • BaseSpace Sequence Hub: Introduction and Analysis Overview

    Recorded Webinar (May 2020) | llumina Technical Support invites you to learn about BaseSpace, Illumina’s cloud-based sequencing data analysis solution. In this presentation, we will discuss how to navigate the BaseSpace dashboard, general BaseSpace functionally, and tips and tricks for successful application and workflow utilization. We will cover the following topics: How to upload/view data in BaseSpace, discussion of major analysis workflows, general overview of how to analyze data on BaseSpace.

    Please note: BaseMount and BaseSpace CLI are alpha software releases; features and functionality may change over time, and support is directly through Illumina software development.


  • Diagnosing Suboptimal Clustering in Nonpatterned Flow Cells

    Learn which run metrics and thumbnail images to monitor during a run to diagnose over clustering of a nonpatterned flow cell.

    8 min


  • Local Run Manager v2 (Research Use Only): Introduction - On- and Off-Instrument

    Recorded Webinar (January 2021) | Local Run Manager is a Windows-based software for Illumina benchtop sequencing data that includes local analysis options as well as run and user management. It will provide an overview of the Research Use Only (RUO) version of Local Run Manager v2 including the off-instrument version and the versions bundled with instrument control software. This webinar does not cover the Dx versions of the software. We cover the following topics: features of Local Run Manager v2, available versions and analysis options, how to set up runs and analyze data, interaction with on-instrument control software and comparison to other Illumina analysis software.