AmpliSeq for Illumina Comprehensive Panel v3 Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for the NextSeq 550 System.

Library Prep

Panel Description

The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair.

Component Number of Pools Concentration Number of Amplicons Average Amplicon Length (bp) Average Library Length (bp)
DNA 2 2X 3781 105 245
RNA 2 5X 867 99 239
Library Prep and Index Kits

Completing the AmpliSeq for Illumina Comprehensive Panel v3 protocol requires the panel and the following library prep and index kit. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.

Number of Samples

The AmpliSeq for Illumina Comprehensive Panel v3 protocol generates one DNA and one RNA library per sample. Samples can be DNA, RNA, or paired. Samples for this protocol are paired. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library is a unique DNA or RNA preparation that has the ends required for sequencing.

Preparing the appropriate number of samples requires the following number and combination of kits.

Number of Samples 24 Samples 96 Samples 384 Samples
Number of Libraries 48 Libraries
(24 DNA, 24 RNA)
192 Libraries
(96 DNA, 96 RNA)
768 Libraries
(384 DNA, 384 RNA)
AmpliSeq for Illumina Comprehensive Panel v3 1 4 16
AmpliSeq Library PLUS For Illumina 2 24-reaction kits 2 96-reaction kits 2 384-reaction kits
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 2 8
cDNA Synthesis 1 1 4

Sequencing Guidelines

Sequence AmpliSeq for Illumina Comprehensive Panel v3 libraries using the following systems and reagent kits.

DNA Samples
System Reagent Kit Samples per Run
MiniSeq System MiniSeq High Output Reagent Kit 3
MiSeq System MiSeq Reagent Kit v3 3
NextSeq System NextSeq Mid Output Kit v2 16
  NextSeq High Output Kit v2 48
RNA Samples
System Reagent Kit Samples per Run
MiniSeq System MiniSeq Mid Output Reagent Kit 24
  MiniSeq High Output Reagent Kit 80
MiSeq System MiSeq Reagent Nano Kit v2 3
  MiSeq Reagent Micro Kit v2 12
  MiSeq Reagent Kit v2 48
  MiSeq Reagent Kit v3 80
Combined DNA and RNA Samples 
System Reagent Kit DNA Samples per Run RNA Samples per Run Combined Samples per Run
MiniSeq System MiniSeq High Output Reagent Kit 3 3 6
MiSeq System MiSeq Reagent v3 3 3 6
NextSeq System NextSeq Mid Output Kit v2 16 16 32
NextSeq High Output Kit v2 48 48 96