This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.
This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
Recorded Webinar (March 2019) | This webinar introduces our line of amplicon products: AmpliSeq for Illumina- compatible with Illumina next-generation sequencing platforms. We will discuss the following topics: available fixed panels, library preparation workflow, the new Design Studio, and and overview of analysis options.
Recorded Webinar (March 2018) | Have a targeted sequencing project in mind? This webinar is targeted toward new and intermediate users looking to design a custom targeted sequencing assay in DesignStudio. We wiill show you how to use the software, and share tips and tricks to create a design for AmpliSeq for Illumina, Nextera Rapid Capture, and TruSeq Targeted RNA Expression.
Upcoming Webinar | Have a customized targeted sequencing project in mind? This webinar is targeted toward new and intermediate users and will focus on using Illumina’s DesignStudio tool to design a custom targeted sequencing assay. We will cover the following subjects: How to use DesignStudio, workflow and how to create a design for: AmpliSeq for Illumina, Nextera Rapid Capture, TruSeq Targeted RNA Expression, and tips and tricks for a successful design.
May 07, 2019 PST
Upcoming Webinar | Does my library look good to sequence? What’s that peak? Join Illumina Technical Support for a webinar on how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover the following topics: how to identify features of an ideal library trace, how to recognize potential issues, and how to prevent potential issues.
Apr 25, 2019 PST