TruSeq Custom Amplicon Low Input Library Prep Kit Contents & Storage

Box 1, Pre-Amplification
Quantity

Reagent

Description

Storage
1

ACP3

Amplicon Control DNA Oligo Pool 3

-25° to -15°C
1

ELE

Extension Ligation Enzyme

-25° to -15°C
1

ELB

Extension Ligation Buffer

-25° to -15°C
1

EDP

Enhanced DNA Polymerase

-25° to -15°C
1

EMM

Enhanced Master Mix

-25° to -15°C
1

2800M

2800M Control DNA

2° to 8°C
Box 2, Pre- and Post-Amplification
Quantity

Reagent

Description

Storage Area
1

SS1

Sample Stabilization Solution 1

2° to 8°C Pre-Amp
1

SPB

Sample Purification Beads

2° to 8°C Pre-Amp
1

OHS2

Oligo Hybridization for Sequencing Reagent 4

2° to 8°C Pre-Amp
1

SW1

Stringent Wash 1

2° to 8°C Pre-Amp
1

RS1

Resuspension Solution 1

Room temperature Pre-Amp
1

LNB1

Library Normalization Beads 1

2° to 8°C Post-amp
Box 3, Post-Amplification
Quantity

Reagent

Description

Storage
1

HT1

Hybridization Buffer

-25° to -15°C
1

LNA1

Library Normalization Additives 1

-25° to -15°C
1

LNW1

Library Normalization Wash 1

-25° to -15°C
1

LNS2

Library Normalization Storage Buffer 2

Room temperature
1

RSB

Resuspension Buffer

Room temperature