BlueFuse Multi Software FAQs

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  • General


  • The following Illumina products are compatible with analysis in the CytoChip and Karyomapping BlueFuse Multi Modules:

    CytoChip Module

    • Infinium CytoSNP-850K
    • Infinium CytoSNP-12
    • Veriseq PGS

    Karyomapping Module

    • Infinium Karyomap-12

    To install and register BlueFuse Multi, download the software from Product Files

    Once you have downloaded and installed the software, send the registration file request to techsupport@illumina.com to receive a registration file to register the software.

    To get your registration codes, select Help | About, and then click Registration on the About dialog.

    Upgrade BlueFuse Multi by running the latest BlueFuse Multi version installer.

    After you have upgraded the BlueFuse Multi Software, you will be prompted to upgrade an existing database the first time you access it. Go through the prompts of typing ‘123’ and click "OK" to upgrade the existing database. 

    Different configurations of views are stored as custom shortcuts. Only administrators can set a shortcut view. 
    1. Select views in the side panel.
    2. Click and drag the boundaries and title bars of the views to configure the sizes and locations on the screen.
    3. Click Tools | Manage Shortcuts.
    4. Enter a name for your custom view and click Add Current.
    5. You can also reorder the list or remove any shortcuts.
    6. Click OK to save the new settings to the database.

     

    Only administrators and supervisors can set a custom report configuration.

    1. Click Tools | Global Configuration | Custom Report Configuration.
    2. Select Experiment Type.
    3. Select the information required in the report. For more information, see the "Report Contents" section in the BlueFuse Multi Software Guide.
    4. Click Save All or Save Current to save the new settings to the database.
  • Database


  • A database stores the information required to run the software and saves the data generated from BlueFuse Multi analysis. Create the database on the local computer drive or on a networked drive.

    Classic Mode:

    1. Click Tools | Change Database Path.
    2. Browse to the directory for the new database. Use the full UNC path that is used for locating databases to improve speeds across the network.
    3. Click Make New Folder, and then name the new database.
    4. Fill in the administrator details.
    5. Click OK.
    6. Import an annotation database

    Server Mode:

    1. Click Tools | Change Database Path.
    2. Select Connect to Database Server.
    3. Enter the Host directory path for the server.
    4. Enter the Port directory. The default is 12345.
    5. Browse to the directory for the new database.
    6. Click Make New Folder, and then name the new database.
    7. Fill in the administrator details.
    8. Click OK.
    9. Import an annotation database.

    Download the annotation database from Product Files.

    To import an annotation database into a BlueFuse Multi database:

    1. In BlueFuse Multi, go to Tools | Import New Annotation
    2. Browse to the downloaded, extracted annotation database file and click Open. 

    In BlueFuse Multi, go to Tools | Change Database Path, and select the top-level directory for the database folder.

    If the database is from an older version of BlueFuse Multi, the software will prompt to perform a database upgrade. Follow the prompts and click "OK" to upgrade the database.  

    BlueFuse Multi software does not specify a maximum database size. The limitation will be the capacity of the drive that hosts the database folder. Please note that increased database size may result in slower performance due to hardware and network limitations. 

    BlueFuse Multi manages a hidden set of folders/files and requires all of them to be present. You may only copy/move the entire database hierarchy along with ALL of the files/subfolders within it. If you delete any individual files or hidden folders BlueFuse Multi may cease to function. In general, and certainly before moving the entire database, we would recommend you first obtain a full back-up.

    1. Select the top-level database folder.
    2. Right-click, and then click Copy.
    3. Paste the folder in the destination location

    If other users are currently using the database, do not copy or move it. Large databases can take a long time to copy. It is recommended to perform the process overnight.

    For VeriSeq PGS, store the original sequencing run folder in a different folder from the database to have a record of all raw data. If necessary, this data can be used to reimport data into BlueFuse Multi.

    1. Click Users | Reset Admin Password.
    2. A new, random password is generated. Click Copy to Clipboard, and email the password to techsupport@illumina.com
    3. Illumina support will return an email with a text string. Enter the text string in the verification box.
    4. Click OK to set the new password.
    5. To change this password after the reset, go to Users | Change Password.