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Contents & Storage
The AmpliSeq for Illumina BRCA protocol requires AmpliSeq for Illumina BRCA Panel (Catalog # 20019168), AmpliSeq for Illumina Library PLUS (Catalog # 20019101, 20019102, or 20019103), and AmpliSeq for Illumina CD Indexes Set A (96 indexes, 96 samples) (Catalog # 20019105). The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.
The AmpliSeq for Illumina BRCA Panel is a 2 oligo pool panel for DNA samples. The following number of kits are required to prepare the appropriate DNA samples. One DNA sample* will prepare one library†.
Number of Samples |
24 Samples |
96 Samples | 384 Samples |
| Number of DNA Libraries | 24 DNA Libraries | 96 DNA Libraries | 384 DNA Libraries |
| AmpliSeq for Illumina BRCA Panel | 1 | 4 | 16 |
| AmpliSeq Library PLUS For Illumina | 1 - 24 reaction kit (20019101) | 1 - 96 reaction kit (20019102) | 1 - 384 reaction kit (20019103) |
| AmpliSeq CD Set A (96 reaction, 96 indexes) | 1 | 1 | 4 |
| cDNA Synthesis | - | - | - |
*A sample is a specimen of nucleic acid from an individual source.
†A library is nucleic acids from a sample after preparation and contains the required ends for sequencing.
AmpliSeq for Illumina BRCA Panel, Store at -25°C to -15°C*
Quantity |
Reagent |
||
| 1 | 5X AmpliSeq BRCA Primer Pool 1 | ||
| 1 | 5X AmpliSeq BRCA Primer Pool 2 |
*Ships at room temperature, but must be stored at -25°C to -15°C
AmpliSeq Library PLUS for Illumina Contents, Store at -25°C to -15°C
Tube Quantity for Kit Configuration
24-reaction |
96-reaction |
384-reaction | Reagent |
| 1 | 4 | 16 | 1X Lib Amp Mix |
| 1 | 1 | 4 | 10X Library Amp Primers |
| 1 | 1 | 4 | DNA Ligase |
| 1 | 1 | 4 | 5X AmpliSeq HiFi Mix |
| 1 | 1 | 4 | FuPa Reagent |
| 1 | 2 | 8 | Low TE** |
| 1 | 1 | 4 | Switch Solution |
**Can be stored at room temperature
AmpliSeq for Illumina CD Indexes Set A, Store at -25°C to -15°C*
Quantity |
Description |
| 1 | AmpliSeq CD Indexes Set A plate (96 indexes, 96 samples) |
*Ships at room temperature, but must be stored at -25°C to -15°C
AmpliSeq Library Equalizer for Illumina
Quantity |
Description |
Storage |
| 1 | Equalizer Beads | 2°C to 8°C |
| 1 | Equalizer Capture | 2°C to 8°C |
| 1 | Equalizer Elution Buffer | 2°C to 8°C |
| 1 | Equalizer Wash Buffer | 15°C to 30°C |
| 1 | Equalizer Primer | 2°C to 8°C |