Nextera Rapid Capture Exome and Expanded Exome Kits Contents & Storage

The Nextera Rapid Capture Exome and Expanded Exome Enrichment kits are packaged in various 8, 24, 48, 72, and 96 sample configurations. The Exome and Expanded Exome kits differ in the oligo contents of Box 4/Oligos box.

Box 1, Store as specified

Reagent

Storage

Description

SPB

2° to 8°C

Sample Purification Beads

SMB

2° to 8°C

Streptavidin Magnetic Beads

ET2

2° to 8°C

Elute Target Buffer 2

ST

15° to 30°C

Stop Tagment Buffer

Box 2, Store at -15° to -25°C

Reagent

Storage

Description

RSB

-15° to -25°C

Resuspension Buffer

TDE1

-15° to -25°C

Tagment DNA Enzyme

TD

-15° to -25°C

Tagment DNA Buffer

EWS

-15° to -25°C

Enrichment Wash Solution

NLM

-15° to -25°C

Library Amp Mix

EHB

-15° to -25°C

Enrichment Hybridization Buffer

HP3

-15° to -25°C

2N NaOH

PPC

-15° to -25°C

PCR Primer Cocktail

NEM

-15° to -25°C

Enrichment Amp Mix

EE1

-15° to -25°C

Enrichment Elution Buffer 1

Box 3, Store Store at -15° to -25°C

Reagent

Storage

Description

Index Primers

-15° to -25°C

E502 to E508*

Index Primers

-15° to -25°C

N701 to N712*

*The indices provided vary per Nextera Rapid Capture Enrichment Kit configuration.

Box 4/Oligos, Store at -15° to -25°C

The Nextera Rapid Capture Exome and Expanded Exome Enrichment kits contain one of the following, depending on the kit type.

Kit

Reagent

Storage

Description

Exome

CEX

-15° to -25°C

Coding Exome Oligos

Expanded Exome

EEX

-15° to -25°C

Expanded Exome Oligos

Index Adapter Replacement Caps Box, Store at 15° to 30°C

Storage

Description

15° to 30°C

i7 Index Tube Caps, Orange

15° to 30°C

i5 Index Tube Caps, White