TruSeq Bovine Parentage Sequencing Panel Contents & Storage

Box 1 - Store in Pre-PCR Area

This box also contains HYP, FPU, and IAP barcode labels.

Quantity

Reagent

Description

Storage
1 ACD1 Amplicon Control DNA 1 -25°C to -15°C
1

ACP1

Amplicon Control DNA Oligo Pool 1

-25°C to -15°C
1

OHS2

Oligo Hybridization for Sequencing Reagent 2

-25°C to -15°C
1

ELM4

Extension Ligation Mix 4

-25°C to -15°C
1

PMM2

PCR Master Mix 2

-25°C to -15°C
1

TDP1

TruSeq DNA Polymerase 1

-25°C to -15°C
1

SW1

Stringent Wash 1

2°C to 8°C
1

UB1

Universal Buffer 1

2°C to 8°C
2° to 8°C
Box 2 - Store in Pre-PCR Area

This box is shipped at room temperature. As soon as you receive your kit, remove LNB1 from box 2 and store at 2°C to 8°C in the post-amplification area. Keep the filter plate in the pre-PCR area at room temperature.

Quantity

Reagent

Description

Storage
    Filter plate with lid Room temperature
1

LNB1

Library Normalization Beads 1

2°C to 8°C
2° to 8°C
Box 3 - Store in Post-PCR Area

This box also contains CLP, LNP, SGP, PAL, and DAL barcode labels.

Quantity

Reagent

Description

Storage
1 HT1 Hybridization Buffer -25°C to -15°C
1

LNA1

Library Normalization Additives 1

-25°C to -15°C
1

LNW1

Library Normalization Wash 1

-25°C to -15°C
1

LNS2

Library Normalization Storage Buffer 2

Room temperature
1

EBT

Elution Buffer with Tris

Room temperature
2° to 8°C
Box 4 - Store in Pre-PCR Area

Quantity

Reagent

Description

Storage
1

BVP1

Bovine Parentage Oligos

-25°C to -15°C
2° to 8°C