TruSeq DNA PCR-Free Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system.

• MiniSeq System
• NextSeq 500 or NextSeq 550 Systems
• HiSeq Series—Use Illumina Experiment Manager to record samples

Index Kits

Completing the TruSeq DNA PCR-Free protocol requires the library prep kit and a compatible index kit.

• TruSeq DNA Single Indexes
• TruSeq DNA CD Indexes
• IDT for Illumina TruSeq DNA UD Indexes

Loading Concentration

After library preparation, denature and dilute libraries to the appropriate loading concentration using the instructions for standard normalized libraries.

• MiniSeq System Denature and Dilute Libraries Guide
• NextSeq System Denature and Dilute Libraries Guide
• HiSeq System Denature and Dilute Libraries Guide

Demo Data Sets

FASTQ, VCF, and BAM files are available for the following HiSeq 2500 data sets. Viewing the data sets requires signing in to BaseSpace Sequence Hub.

• TruSeq DNA library
• TruSeq DNA PCR-Free 350 bp library
• TruSeq DNA PCR-Free 550 bp library
• TruSeq Nano DNA 350 bp library
• TruSeq Nano DNA 550 bp library