Box 1 (Pre-Amp) Library Prep
DNA/RNA bundle customers receive one box. DNA kit customers do not receive this box.
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
FSM |
First Strand Synthesis Mix |
-25°C to -15°C |
| 1 |
SSM |
Second Strand Mix |
-25°C to -15°C |
| 1 |
EPH |
Elute, Prime, Fragment High Mix 3 |
-25°C to -15°C |
| 1 |
RVT |
Reverse Transcriptase |
-25°C to -15°C |
Box 2 (Pre-Amp) Library Prep
| Quantity |
Reagent |
Description |
Storage |
|---|
| 2 |
UMI1 |
UMI Adapters v1 |
-25°C to -15°C |
Box 3 (Pre-Amp) Library Prep
| Quantity |
Reagent |
Description |
Storage |
|---|
| 2 |
ERA1-A |
End Repair A-tailing Enzyme Mix 1 |
-25°C to -15°C |
| 2 |
ERA1-B |
End Repair A-tailing Buffer 1 |
-25°C to -15°C |
| 2 |
ALB1 |
Adapter Ligation Buffer 1 |
-25°C to -15°C |
| 2 |
LIG3 |
DNA Ligase 3 |
-25°C to -15°C |
| 2 |
SUA1 |
Short Universal Adapters 1 |
-25°C to -15°C |
| 2 |
STL |
Stop Ligation Buffer |
-25°C to -15°C |
| 2 |
EPM |
Enhanced PCR Mix |
-25°C to -15°C |
Box 4 (Pre-Amp) Library Prep
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
RSB |
Resuspension Buffer |
2°C to 8°C or -25°C to -15°C |
| 2 |
SPB |
Sample Purification |
2°C to 8°C |
| 1 |
TEB |
TE Buffer |
2°C to 8°C |
Box 5 (Pre-Amp) Library Prep Unique PCR Index Primer Mixes
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
UP01 |
Unique Index Mix Primer 01 |
-25°C to -15°C |
| 1 |
UP02 |
Unique Index Mix Primer 02 |
-25°C to -15°C |
| 1 |
UP03 |
Unique Index Mix Primer 03 |
-25°C to -15°C |
| 1 |
UP04 |
Unique Index Mix Primer 04 |
-25°C to -15°C |
| 1 |
UP05 |
Unique Index Mix Primer 05 |
-25°C to -15°C |
| 1 |
UP06 |
Unique Index Mix Primer 06 |
-25°C to -15°C |
| 1 |
UP07 |
Unique Index Mix Primer 07 |
-25°C to -15°C |
| 1 |
UP08 |
Unique Index Mix Primer 08 |
-25°C to -15°C |
| 1 |
UP09 |
Unique Index Mix Primer 09 |
-25°C to -15°C |
| 1 |
UP10 |
Unique Index Mix Primer 10 |
-25°C to -15°C |
| 1 |
UP11 |
Unique Index Mix Primer 11 |
-25°C to -15°C |
| 1 |
UP12 |
Unique Index Mix Primer 12 |
-25°C to -15°C |
| 1 |
UP13 |
Unique Index Mix Primer 13 |
-25°C to -15°C |
| 1 |
UP14 |
Unique Index Mix Primer 14 |
-25°C to -15°C |
| 1 |
UP15 |
Unique Index Mix Primer 15 |
-25°C to -15°C |
| 1 |
UP16 |
Unique Index Mix Primer 16 |
-25°C to -15°C |
Box 6 (Post-Amp) Enrichment
| Quantity |
Reagent |
Description |
Storage |
|---|
| 2 |
TCB1 |
Target Capture Buffer 1 |
2°C to 8°C |
| 2 |
SMB |
Streptavidin Magnetic Beads |
2°C to 8°C |
| 2 |
HP3 |
2N NaOH |
2°C to 8°C |
| 2 |
ET2 |
Elution Target 2 |
2°C to 8°C |
| 2 |
LNB1 |
Library Normalization Beads 1 |
2°C to 8°C |
| 2 |
LNW1 |
Library Normalization Wash 1 |
2°C to 8°C |
| 1 |
RSB |
Resuspension Buffer |
2°C to 8°C or -25°C to -15°C |
| 2 |
SPB |
Sample Purification Beads |
2°C to 8°C |
| 2 |
LNS1 |
Library Normalization Storage 1 |
15°C to 30°C |
Box 7 (Post-Amp) Enrichment
| Quantity |
Reagent |
Description |
Storage |
|---|
| 2 |
TCA1 |
Target Capture Additive 1 |
-25°C to -15°C |
| 1 |
EEW2 |
Enhanced Enrichment Wash 2 |
-25°C to -15°C |
| 3 |
EE2 |
Enrichment Elution 2 |
-25°C to -15°C |
| 2 |
EPM |
Enhanced PCR Mix |
-25°C to -15°C |
| 2 |
PPC3 |
PCR Primer Cocktail 3 |
-25°C to -15°C |
| 1 |
LNA1 |
Library Normalization Additives 1 |
-25°C to -15°C |
Box 8 (Post-Amp) Enrichment
DNA/RNA bundle customers receive one box. DNA kit customers receive two of this box.
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
OPD2 |
Oncology DNA Probes Master Pool 2 |
-25°C to -15°C |
Box 9 TruSight Oncology 500 Kit Content Set
DNA/RNA bundle customers receive one box. DNA kit customers do not receive this box.
| Quantity |
Reagent |
Description |
Storage |
|---|
| 1 |
OPR1 |
Oncology RNA Probes Master Pool |
-25°C to -15°C |