The VeriSeq PGS Kit contains two SurePlex DNA Amplification System Kits, one VeriSeq Library Prep Kit– PGS, one VeriSeq Index Kit– PGS, and four MiSeq Reagent Kits v3– PGS. Components are shipped on dry ice unless specified otherwise below. As soon as you receive your kit, store the kit components at the specified temperature in designated pre-amplification and post-amplification areas.
SurePlex DNA Amplification System Kit, Store at -25°C to -15°C
| Reagent |
| Cell Extraction Buffer |
| Extraction Enzyme Dilution Buffer |
| Cell Extraction Enzyme |
| SurePlex Pre-Amp Enzyme |
| SurePlex Amplification Buffer |
| SurePlex Amplifcation Enzyme |
VeriSeq Library Preparation Kit–PGS
Box 1, Freeze until use
| Reagent |
Description |
Storage |
| ATM |
Amplicon Tagment Mix, 96 RXN |
-25°C to -15°C |
| TD |
Tagment DNA Buffer |
-25°C to -15°C |
| NPM |
Nextera PCR Master Mix |
-25°C to -15°C |
| RSB |
Resuspension Buffer |
-25°C to -15°C |
| LNA1 |
Library Normalization Additives 1 |
-25°C to -15°C |
| LNW1 |
Librart Normalization Wash 1 |
2°C to 8°C |
| HT1 |
Hybridization Buffer |
-25°C to -15°C |
Box 2, Refrigerate until use
| Reagent |
Description |
Storage |
| NT |
Neutralize Tagment Buffer |
Room temperature |
| LNB1 |
Library Normalization Buffer |
2°C to 8°C |
| LNS1 |
Library Normalization Storage Buffer 1 |
Room temperature |
VeriSeq Index Kit– PGS (96 samples)
| Quantity |
Component |
Storage |
| 4 tubes |
Index Primers, S503, S504 |
-25°C to -15°C |
| 12 tubes |
Index Primers, N701 to N712 |
-25°C to -15°C |
MiSeq Reagent Kit v3–PGS
Box 1
| Quantity |
Component |
Storage |
| 1 |
Reagent Cartridge |
-25°C to -15°C |
| 1 |
HT1 |
-25°C to -15°C |
Box 2
| Quantity |
Component |
Storage |
| 1 |
PR2 Bottle |
2°C to 8°C |
| 1 |
MiSeq Flow Cell |
2°C to 8°C |