IDT for Illumina Nextera DNA UD Indexes Files for Illumina Experiment Manager

Use the following sample sheet templates with IDT for Illumina Nextera DNA UD Indexes.

Dual-index sequencing on a paired-end flow cell follows 1 of 2 workflows, depending on the system:

Workflow A — The Index 2 Read is performed before Read 2 resynthesis, so the Index 2 (i5) adapter is sequenced on the forward strand. Workflow A is performed on the NovaSeq, MiSeq, HiSeq 2500, and HiSeq 2000. 

Workflow B — The Index 2 Read is performed after Read 2. Workflow B is performed on the MiniSeq, NextSeq, HiSeq 4000, and HiSeq 3000.

For more information, see the Indexed Sequencing Overview Guide.

Files

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