AmpliSeq for Illumina Immune Response Panel Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system:

Library Prep

Panel Description

The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing assay for quantitating expression of cancer biomarkers in 395 genes involved in tumor-immune system interactions.

Number of Pools Concentration Number of Amplicons Average Amplicon Length (bp) Average Library Length (bp)
1 5X 398 106 246
Library Prep and Index Kits

Completing the AmpliSeq for Illumina Immune Response Panel protocol requires the panel and the following library prep kit and index kit.

Number of Samples

For each RNA sample, AmpliSeq for Illumina Immune Response Panel generates one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.

Preparing the appropriate number of samples requires the following number and combination of kits. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.

Number of Samples 24 Samples 96 Samples 384 Samples
Number of Libraries 24 Libraries 96 Libraries 384 Libraries
AmpliSeq for Illumina Immune Response Panel 1 4 16
AmpliSeq Library PLUS For Illumina 1 24-reaction kit 1 96-reaction kit 1 384-reaction kit
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 1 4
cDNA Synthesis 1 1 4

Sequencing Guidelines

Sequence AmpliSeq for Illumina Immune Response Panel libraries using the following systems and reagent kits:

System Reagent Kit Samples per Run
MiniSeq System MiniSeq Mid Output Reagent Kit 8
MiniSeq High Output Reagent Kit 24
MiSeq System MiSeq Reagent Micro Kit v2 4
MiSeq Reagent Kit v2 16
MiSeq Reagent Kit v3 24
NextSeq System NextSeq Mid Output v2 Kit 96