Nextera DNA Exome Contents & Storage

Nextera DNA Exome Kit (24 Samples)

Nextera DNA Library Prep for Enrichment
Box 1
Quantity Reagent Description Storage
1 SPB Sample Purification Beads 2°C to 8°C
1 ST Stop Tagment Buffer 15°C to 30°C
Box 2, Store at -25°C to -15°C
Quantity Reagent Description
1 RSB Resuspension Buffer
1 TDE2 Tagment DNA Enzyme 2
1 LAM Library Amplification Mix
1 TD Tagment DNA Buffer
Nextera DNA for Enrichment
Box 1, Store at -25°C to -15°C
Quantity Reagent Description
1 COT Blocker
1 EAM Enrichment Amplification Mix
1 EE1 Enrichment Elution Buffer 1
2 EEW Enhanced Enrichment Wash Solution
1 EHB1 Enrichment Hybridization Buffer 1
1 HP3 Finished Reagent
1 PPC PCR Primer Cocktail
Box 2, Store at 2°C to 8°C
Quantity Reagent Description
1 EHB2 Enrichment Hybridization Buffer
1 ET2 Elute Target Buffer 2
4 SMB Strepravidin Magnetic Beads
Nextera DNA CD Indexes, Store at -25°C to -15°C
Quantity Reagent Description
1 H503 DNA Adapter
1 H505 DNA Adapter
1 H506 DNA Adapter
1 H517 DNA Adapter
1 H710 DNA Adapter
1 H705 DNA Adapter
1 H706 DNA Adapter
1 H707 DNA Adapter
1 H711 DNA Adapter
1 H714 DNA Adapter
Index Adapter Replacement Caps, Store at 15°C to 30°C
Description
i7 Index Tube Caps, Orange
i5 Index Tube Caps, White
Exome Panel (45mb), Store at -25°C to -15°C
Quantity Reagent Description
4 CEX Coding Exome Oligos

Nextera DNA Exome Kit (96 Samples)

Nextera DNA Library Prep for Enrichment
Box 1
Quantity Reagent Description Storage
3 SPB Sample Purification Beads 2°C to 8°C
1 ST Stop Tagment Buffer 15°C to 30°C
Box 2, Store at -25°C to -15°C
Quantity Reagent Description
1 RSB Resuspension Buffer
1 TDE2 Tagment DNA Enzyme 2
2 LAM Library Amplification Mix
2 TD Tagment DNA Buffer
Nextera DNA for Enrichment
Box 1, Store at -25°C to -15°C
Quantity Reagent Description
1 COT Blocker
1 EAM Enrichment Amplification Mix
1 EE1 Enrichment Elution Buffer 1
2 EEW Enhanced Enrichment Wash Solution
1 EHB1 Enrichment Hybridization Buffer 1
1 HP3 Finished Reagent
1 PPC PCR Primer Cocktail
Box 2, Store at 2°C to 8°C
Quantity Reagent Description
1 EHB2 Enrichment Hybridization Buffer
1 ET2 Elute Target Buffer 2
4 SMB Strepravidin Magnetic Beads
Nextera DNA CD Indexes, Store at -25°C to -15°C
Quantity Description
1 96 Dual Adapter Plate
Exome Panel (45mb), Store at -25°C to -15°C
Quantity Reagent Description
4 CEX Coding Exome Oligos