Nextera Rapid Capture Exome and Expanded Exome Enrichment Kits Product Files

Changes introduced in the Nextera Rapid Capture Exome v1.2 manifest files.

Nextera Rapid Capture Exome target region intervals that do not overlap the Nextera Rapid Capture Exome target region. Exome has 4.1 MB unique intervals: 336,295 intervals across 205,598 exome targeted regions, as determined by base overlap.

For example, comparing an exome product region at Chr 1 position 300–500 with an expanded exome region at Chr 1 position 305–495 results in a 191 bp overlap and two 5 bp unique regions for exome.

Nextera Rapid Capture Expanded Exome target region intervals that do not overlap the Nextera Rapid Capture Exome target regions. A total of 29 MB are unique to expanded exome: 50,257 intervals across 47,273 Expanded Exome targeted regions (determined by base overlap only).

For example, comparing an exome region at Chr 1 position 300–500 with an expanded exome region at Chr 1 position 305–495 results in a 191 bp overlap and two 5 bp unique regions for the exome product.

Nextera Rapid Capture Exome targeted region intervals that overlap Nextera Rapid Capture Expanded Exome targeted regions. The total overlap is 33 MB between the two products: 189,522 overlapping intervals across 188,621 exome targeted regions (determined by base overlap).

For example, comparing exome region at Chr 1 position 300–500 with an expanded exome region at Chr 1 position 305–495 results in a 191 bp overlap and two 5 bp unique regions for the exome product.

Demo dataset to validate installation of the enrichment workflow in HiSeq Analysis Software (HAS). It includes a run folder that contains 10% of tiles from one lane of reads from the Coriell Sample, GM10861, enriched using the Nextera Rapid Capture Exome Kit with a plexity of 12.

Use the command $tar -zxvf NexteraRapidCapture_DemoData to unzip the *tar.gz folder.

Human reference genome hg19 from UCSC for the HiSeq Analysis Software.