TruSeq Genotype Ne Contents & Storage

Library Prep Reagents

Box 1 - DNA Ampicon Assay v2 (16 Samples), Store at -25°C to -15°C
Quantity Reagent Description
1 ACP3 Amplicon Control Oligo Pool 3
1 EDP Enhanced DNA Polymerase
1 ELB Extension-Ligation Buffer
1 ELE Extension-Ligation Enzyme
1 EMM Enhanced Master Mix

Box 1 also contains the HYP barcode label.

Box 2 - DNA Amplicon Assay v2 (16 Samples)
Quantity Reagent Description Storage
1 LNB1 Library Normalization Beads 2°C to 8°C
1 OHS2 Oligo Hybridization for Sequencing Reagent 2 2°C to 8°C
1 RS1 Resuspension Solution 1 15°C to 30°C
1 SPB Sample Purification Beads 2°C to 8°C
1 SS1 Sample Stabilization Solution 1 2°C to 8°C
1 SW1 Stringent Wash 1 2°C to 8°C
Box 3 - DNA Amplicon Assay v2 (16 Samples) (Pre-PCR)
Quantity Reagent Description Storage
1 HT1 Hybridization Buffer -25°C to -15°C
1 LNA1 Library Normalization Additives 1 -25°C to -15°C
1 LNS2 Library Normalization Storage Buffer 2 15°C to 30°C
2 LNW1 Library Normalization Wash 1 2°C to 8°C
2 RSB Resuspension Buffer 15°C to 30°C

Box 3 also contains the CLP, LNP, SGP, PAL, and DAL barcode labels.

Index Adapters

Nextera XT Index Kit v2 Set A (96 Indexes, 192 Samples), Store at -25°C to -15°C
Quantity Reagent
8 tubes Index Adapters S502, S503, S505–S508, S510, and S511
12 tubes Index Adapters N701–N707, N710–N712, N714, and N715
Nextera XT Index Kit v2 Set B (96 Indexes, 192 Samples), Store at -25°C to -15°C
Quantity Reagent
8 tubes Index Adapters S502, S503, S505–S508, S510, and S511
12 tubes Index Adapters N716, N718–N724, and N726–N729
Nextera XT Index Kit v2 Set C (96 Indexes, 192 Samples), Store at -25°C to -15°C
Quantity Reagent
8 tubes Index Adapters SS513, S515–S518, and S520–S522
12 tubes Index Adapters N701–N707, N710–N712, N714, and N715
Nextera XT Index Kit v2 Set D (96 Indexes, 192 Samples), Store at -25°C to -15°C
Quantity Reagent
8 tubes Index Adapters S513, S515–S518, and S520–S522
12 tubes Index Adapters N716, N718–N724, and N726–N729

Sequencing Reagents

MiniSeq Reagents
Quantity Reagent Storage
1 MiniSeq Reagent Cartridge (150 cycles) -25°C to -15°C
1 MiniSeq Flow Cell 2°C to 8°C
1 Hybridization Buffer (HT1) -25°C to -15°C
NextSeq Reagents
Quantity Reagent Storage
1 NextSeq 500 High Output Reagent Cartridge v2 (150 cycles) -25°C to -15°C
1 NextSeq 500 High Output Flow Cell v2 2°C to 8°C
1 NextSeq 500/550 Buffer Cartridge -25°C to -15°C
1 NextSeq Accessory Box v2 -25°C to -15°C
MiSeq Reagent Kit v3 - Box 1, Store at -25°C to -15°C
Quantity Reagent
1 MiSeq v3 Reagent Tray (150 cycles, PE)
1 Hybridization Buffer (HT1)
MiSeq Reagent Kit v3 - Box 2, Store at 2°C to 8°C
Quantity Reagent
1 PE MiSeq Flow Cell
1 Incorporation Buffer (PR2)