TruSeq RNA Access Library Prep Kit Training

This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

Recorded Webinar (October 2021) | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.

Recorded Webinar (August 2021) | Part 1 of a 3-Part RNA-Seq Webinar Series. This session will review sample preparation kit options for RNA input.  An introduction to the new Illumina Stranded mRNA Ligation, Illumina Stranded Total RNA Ligation with Ribozero Plus, and Illumina RNA prep with Enrichment Tagmentation workflows will be provided along with a comparison to established Illumina kits. This webinar is targeted towards both new and experienced users and will cover the following topics: key considerations when planning RNA sequencing experiments, how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.

Recorded Webinar (September 2021) | In the second part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, Illumina Stranded mRNA Ligation, TruSeq Stranded Total RNA, Illumina Stranded Total RNA Ligation and Illumina RNA prep with Enrichment Tagmentation. We will cover the following topics: bead handling, quantification methods, library QC and troubleshooting.

After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

By the end of this course you will learn what the TruSeq RNA Access Library Prep protocol is, how the protocol works, what is needed to successfully complete the protocol, the various steps in the sample preparation workflow, and how to analyze TruSeq RNA Access Library Prep data.