| Software | Library Types | Additional Information |
|---|---|---|
| DRAGEN TruSight Oncology 500 Analysis Software v1.1 | DNA only DNA and RNA RNA only |
|
| Local Run Manager TruSight Oncology 500 Analysis Module | DNA only DNA and RNA RNA only |
Requires NextSeq Control Software (NCS) v4.0.1 and later. |
| TruSight Oncology 500 Local App v2.2 |
DNA only DNA and RNA RNA only |
|
| TruSight Oncology 500 Local App v2.1 | DNA only DNA and RNA RNA only |
|
| TruSight Oncology 500 Local App v2 | DNA only DNA and RNA RNA only |
|
| TruSight Oncology 500 Local App v1 | DNA only | Due to software constraints, run TruSight Oncology 500 Local App v1 for DNA analysis separately from the Trusight Tumor 170 Local App for RNA analysis. |
| TruSight Tumor 170 Local App | RNA only |
For optimal coverage, use the following guidelines for the number of libraries per sequencing run.
| Library Type | Minimum | Maximum |
|---|---|---|
| DNA-only | 3 | 8 |
| DNA and RNA | 3 | 8 DNA and 8 RNA |
| RNA-only | 3 | 16 |
| Read/Index | Number of cycles |
|---|---|
| Read 1 | 101 |
| Index 1 | 8 |
| Index 2 | 8 |
| Read 2 | 101 |