HiSeq 3000 Documentation

  • Supporting Documents



  • Read 1 Primer Rehybridization on a HiSeq 3000/4000 Flow Cell

    Instructions for performing Read 1 primer rehybridization on a HiSeq 3000/4000 flow cell using the HiSeq 3000/4000 cBot Multi-Primer Rehybridization Kit.


    < 1 MB

    Feb 8, 2016

  • Cluster Optimization Overview

    Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

    View Online Help

    Apr 26, 2021

  • Indexed Sequencing Overview

    The sequencing process for single- and dual-indexed runs on Illumina instruments.

    View Online Help

    Apr 26, 2021

  • Security and Networking

    Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

    It is strongly recommended that you review this documentation to keep your instrument secure.

    View Online Help

    Jan 12, 2024