HiSeq 1500 Compatible Products

Cluster Generation

For high output modes (HiSeq v4 and TruSeq v3), cluster the flow cell on the cBot 2 System or cBot System before sequencing on the HiSeq 1500 System.

Using the HiSeq Rapid Duo cBot Sample Loading Kit to load a different library into each lane of the rapid flow cell also requires clustering on the cBot 2 or cBot. Otherwise, Rapid Run mode on the HiSeq 1500 System performs both cluster generation and sequencing.

Sequencing Primers for Dual-Indexed Libraries

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 1500:

• Any Nextera libraries with any number of indexes. Use the single-read (SR) box with SR flow cells and the paired-end (PE) box with PE flow cells.
• TruSeq HT libraries when performing a dual-indexed run on an SR flow cell.

For more information, see HiSeq 1500 Primers.

Run Components

Use the compatible version of HiSeq Control Software (HCS) and Real-Time Analysis (RTA) software for your kit. For kit details, see HiSeq 1500 High Output Kits or HiSeq 1500 Rapid Run Kits.

High Output

Rapid Run

* Also requires Version R recipes and cBot software v1.5, or later.

Additional Primers