NextSeq 500 Support


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Library Prep and Array Kit Selector

Determine the best kit for your project type, starting material, and method or application.

Local Run Manager Module Selector

Find the analysis modules compatible with your instrument and Local Run Manager configuration.

Sequencing Coverage Calculator

Determine reagents and sequencing runs for your desired coverage.


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Maximize the effectiveness of your Illumina system, train new employees, or learn the latest techniques and best practices. We offer support webinars, online courses, expert video tips, and instructor-led trainings.

Featured Training
Preparing Runs with BaseSpace Sequence Hub

Demo of how to use the BaseSpace Sequence Hub Prep tab to enter sample information and parameters for your sequencing run.

Sequencing Considerations for Low Diversity Libraries

Visualizing base diversity in Sequencing Analysis Viewer (SAV) and strategies to optimize low diversity sequencing data.

Local Run Manager v2 (RUO): Introduction - On- and Off-Instrument

Overview of software features, available versions, run setup steps, and comparison to other Illumina analysis software.