NovaSeq X Plus Compatible Products

Sample Information

Choose from the following options to record information about your samples, such as sample ID and which index adapters are used for pooling. 

  • If connected to the cloud, you can use Run Planning in BaseSpace Sequence Hub. 
  • If connected to your local network, you can use the sample sheet v2 template available from the remote control software. 
  • If not connected to your local network, you can use the the sample sheet generated by a completed run. 

Data Analysis

Use Sequencing Analysis Viewer or BaseSpace Sequence Hub to monitor real-time quality metrics during a run.

To analyze sequencing data, choose from the following options:

The NovaSeq X Plus Sequencing System performs secondary analysis using the on-intsrument Illumina DRAGEN BIO-IT Platform server. Refer to the NovaSeq X Plus Product Documentation for more information.

Reference Genomes

On-instrument Reference Genomes
Genome Name Display Name Species GTF Available
hg19_alt_masked+cnv+hla+rna_v2 Homo sapiens [UCSC] hg19 Alt Masked v2 Homo sapiens [Human] Yes
hg19_alt_masked_graph+cnv+hla+rna_v2 Homo sapiens [UCSC] hg19 Alt Masked v2 Graph Homo sapiens [Human] Yes
hs37d5+cnv+rna Homo sapiens [NCBI] hs37d5 Homo sapiens [Human] Yes
hs37d5_graph_based Homo sapiens [NCBI] hs37d5 Graph Homo sapiens [Human] Yes
hg38_alt_masked+cnv+hla+rna_v2 Homo sapiens [1000 Genomes] hg38 Alt Masked v2 Homo sapiens [Human] Yes
hg38_alt_masked_graph+cnv+hla+rna_v2 Homo sapiens [1000 Genomes] hg38 Alt Masked v2 Graph Homo sapiens [Human] Yes
hg38-noalt-with-decoy Homo sapiens [1000 Genomes] hg38 No Alt Haps Homo sapiens [Human] Yes
A_Thaliana A. thaliana [TAIR] TAIR10 Arabidopsis Thaliana No
eschColi_K12_1 E. coli [NCBI] K12 MG1655 Escherichia Coli [Strain K12] No
mm10 Mus musculus [UCSC] mm10 Mus Musculus [House Mouse] Yes
phix Microvirus Escherichia virus phiX174 [NCBI]PhiX PhiX No
rn6 Rattus norvegicus [UCSC] rn6 Rattus Norvegicus [Norway Rat] Yes
Rhodobacter_sphaeroides_2.4.1_12905 R.sphaeroides [NCBI] 2.4.1 Rhodobacter Sphaeroides [Bacterium] No
Bacillus_cereus_ATCC_10987 B. cereus [NCBI] ATCC_10987 Bacillus Cereus [Bacterium] No
WBcel235 C. elegans [Ensembl] WBcel235 Caenorhabditis Elegans [Roundworm] No
eschColi_K12_1 E.Coli Strain K12 Escherichia Coli [Strain K12] No