In this video, an Illumina Field Applications Scientist discusses the key steps in the Nextera library preparation process and provides some tips and tricks on how to achieve optimal results. Note that the best practices presented in this video do not apply to the Nextera™ DNA Flex Library Prep Kit.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
Recorded Webinar (October 2021) | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.
By the end of this course, you will be able to identify the Nextera XT DNA kits, identify how the protocol works, describe what is needed to complete the protocol, and define the various steps in the library preparation workflow.
Recorded Webinar (April 2017) | Join Illumina Technical Support for a presentation and discussion on the Nextera XT sample preparation process. This session reviews the following topics: Nextera XT protocol, best practices for critical steps, and a comparison of the Nextera XT kit to the Nextera DNA kit, focusing on the applications recommended with each kit.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.