TruSeq Stranded Total RNA Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system.

• NextSeq 500 or NextSeq 550 Systems
• NovaSeq 6000 System
• HiSeq Series—Use Illumina Experiment Manager to record samples

Index Kits

Completing the TruSeq Stranded Total RNA protocol requires the library prep kit and a compatible index kit.

• TruSeq RNA Single Indexes
• TruSeq RNA CD Indexes
• IDT for Illumina TruSeq RNA UD Indexes

Loading Concentration

After library preparation, denature and dilute libraries to the appropriate loading concentration using the instructions for standard normalized libraries.

• NextSeq System Denature and Dilute Libraries Guide
• HiSeq System Denature and Dilute Libraries Guide
• NovaSeq 6000 Sequencing System Guide