HiSeq 2500 High Output Kits
HiSeq 2500 System
High Output Kits
Catalog IDs: PE-401-4001, GD-401-4001, FC-401-4002, FC-401-4003, PE-401-3001, GD-401-3001, FC-401-3001, FC-401-3002
Kit Requirements
Performing a high output run requires one of the following kit combinations, depending on run mode. All kits are single-use.
- HiSeq v4 mode—HiSeq Cluster Kit v4 and HiSeq SBS Kit v4
- TruSeq v3 mode—TruSeq Cluster Kit v3 and TruSeq SBS Kit v3
The cluster kits are available in available in paired-end (PE) and single-read (SR) versions. Both versions contain clustering reagents for the cBot 2 or cBot System and indexing and paired-end reagents for the HiSeq 2500 System. The SBS kits include sequencing reagents to sequence one flow cell on the HiSeq 2500 System.
For other system requirements, dual indexing considerations, and compatible products, see HiSeq 2500 Requirements & Compatibility.
Contents and Storage
When you receive your kits, promptly store kit components at the indicated temperatures to ensure proper performance:
HiSeq Cluster Kit v4
HiSeq Cluster Kit v4 reagents are shipped in two boxes on dry ice. A flow cell, manifold, and accessories are also included.
Cluster Kit Box 1, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
cBot Reagent Plate |
PE kit—cBot PE reagent plate SR kit—cBot SR reagent plate |
PE Cluster Kit Box 2, Store at -25°C to -15°C
Quantity |
Kit Component |
Description |
1 |
AMS |
Fast Amplification Mix, High Output |
1 |
FDR |
Fast Denaturation Reagent, High Output (contains formamide) |
1 |
FPM |
Fast Amplification Premix, High Output |
1 |
FRM |
Fast Resynthesis Mix, High Output |
1 |
FLM2 |
Fast Linearization Mix 2, High Output |
1 |
HP11 |
Read 2 Primer Mix, High Output |
1 |
HP12 |
Index i7 Primer Mix, High Output |
SR Cluster Kit Box 2, Store at -25°C to -15°C
Quantity |
Kit Component |
Description |
1 |
FDR |
Fast Denaturation Reagent, High Output |
1 |
HP12 |
Index 1 (i7) Primer Mix, High Output |
1 |
HP9 |
Index 2 (i5) Primer Mix, High Output |
Accessory Kit
Quantity |
Accessory |
Purpose |
6 |
Flow cell gaskets |
Replace flow cell gaskets between runs. Set contains 8-port gaskets (4) and 10-port gaskets (2). |
8 |
Funnel bottle caps |
Replace SBS reagent bottle caps before loading reagents. |
HiSeq SBS Kit v4
HiSeq SBS Kit v4 reagents are shipped in two boxes. Reagents are sensitive to light. Store in a dark location away from light.
SBS Kit Box 1
Quantity |
Reagent |
Description |
Storage Temperature |
1 |
PW1 |
Wash Buffer |
-25°C to 30°C |
1 |
SB1 |
High Salt Buffer |
2°C to 8°C |
2 |
SB2 |
Incorporation Buffer |
2°C to 8°C |
1 |
SB3 |
Cleavage Buffer |
2°C to 8°C |
SBS Kit Box 2, Store at -25°C to -15°C
Quantity |
Reagent |
Storage Temperature |
Description |
1 |
IRM |
-25°C to -15°C |
Incorporation Reagent Master Mix |
1 |
CRM |
-25°C to -15°C |
Cleavage Reagent Mix |
1 |
USM |
-25°C to -15°C |
Universal Scan Mix |
TruSeq Cluster Kit v3
TruSeq Cluster Kit v3 reagents are shipped in four boxes on dry ice. A flow cell, manifold, and accessories are also included.
PE Cluster Kit Box 1, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
HP3 |
Denaturation Solution |
1 |
HT2 |
Wash Buffer |
1 |
RMR |
Resynthesis Mix |
1 |
AT2 |
100% Formamide |
1 |
APM2 |
Amplification Premix |
1 |
AMX2 |
Amplification Mix 2 |
1 |
LMX2 |
Linearization Mix 2 |
1 |
BMX |
Blocking Mix |
1 |
HP7* |
Read 2 Sequencing Primer Mix |
* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.
PE Cluster Kit Box 2, Store at -25°C to -15°C
Quantity |
Kit Component |
Description |
1 |
cBot Reagent Plate |
cBot PE reagent plate |
1 |
HT1 |
Hybridization Buffer |
SR Cluster Kit Box, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
cBot Reagent Plate |
cBot SR reagent plate |
1 |
HT1 |
Hybridization Buffer |
Multiplex Sequencing Primer Box, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
HP3 |
Denaturation Solution |
1 |
HT2 |
Wash Buffer |
1 |
HP8* |
Index Sequencing Primer |
* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.
Accessory Kit
Quantity |
Component |
Purpose |
4 |
Flow cell gaskets |
Replace the flow cell gaskets between runs. |
8 |
Funnel bottle caps |
Replace SBS reagent bottle caps before loading reagents. |
1 |
ICB instrument tube |
Divide ICB for Read 2. |
TruSeq SBS Kit v3
TruSeq SBS Kit v3 reagents are shipped in two boxes on dry ice.
Reagents are labeled with a three-letter acronym plus a numeric designator that indicates the kit size. For example, if you are using CMR-50, the CMR was provided in a 50-cycle kit.
Box 1
Quantity |
Reagent |
Description |
Storage Temperature |
1 |
PW1 |
Wash Buffer |
-25°C to 30°C |
1 |
ICB |
Incorporation Mix Buffer |
2°C to 8°C |
1 |
SB1 |
High Salt Buffer |
2°C to 8°C |
2 |
SB2 |
Incorporation Wash Buffer |
2°C to 8°C |
1 |
SB3 |
Cleavage Buffer |
2°C to 8°C |
Box 2, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
CMR |
Cleavage Mix Reagent |
1 |
SRE |
Enhanced Scan Mix Reagent |
1 |
EDP |
Enhanced DNA Polymerase |
| 4—200-cycle kit 1—50-cycle kit |
LFN36 |
Long Read Nucleotide Mix |