HiSeq 2500 Primers

Sequencing Primers

Some dual-indexed libraries require sequencing primers in the TruSeq Dual Index Sequencing Primer Box. These primers replace primers provided in the reagent kits:

• Read 1 Sequencing Primer—Load HP10 on the cBot 2 or cBot System instead of HP6.
• Index 1 Read Sequencing Primer—Load HP12 instead of HP8.
• Index 2 Read Sequencing Primer—For single-read flow cells, load HP9. For paired-end flow cells, load RMX from the TruSeq PE Cluster Kit v3.
• Read 2 Sequencing Primer—Load HP11 instead of HP7.

For information on compatible libraries, see HiSeq 2500 Requirements & Compatibility. For clustering and sequencing instructions, see the cBot 2 System Guide or cBot System Guide and the HiSeq 2500 System Guide.

Contents and Storage

TruSeq Dual Index Sequencing Primer Box, Single-Read, Store at -25° to -15°C

TruSeq Dual Index Sequencing Primer Box, Paired-End, Store at -25° to -15°C