HiSeq 2500 Rapid Run Kits
HiSeq 2500 System
Rapid Run Kits
Catalog IDs: PE-402-4002, GD-402-4002, FC-402-4022, FC-402-4021, FC-402-4023, CT-403-2001
Kit Requirements
Performing a rapid run requires a single-use HiSeq Rapid Cluster Kit v2 and a single-use HiSeq Rapid SBS Kit v2. The kits contain a flow cell and reagents for clustering and sequencing on the HiSeq 2500 System in Rapid Run mode.
- The cluster kit is available in available in paired-end (PE) and single-read (SR) versions.
- The SBS kit is available in three sizes: 500-, 200-, and 50-cycles.
Alternatively, you can use the cBot 2 or cBot System to load two libraries onto the flow cell, one in each lane. This option requires the HiSeq Rapid Duo cBot Sample Loading Kit in addition to the HiSeq Rapid Cluster Kit v2 and HiSeq Rapid SBS Kit v2. The HiSeq Rapid Duo cBot Sample Loading Kit performs template hybridization and first extension on the cBot 2 or cBot. Clustering is then completed on the HiSeq 2500.
For other system requirements, dual indexing considerations, and compatible products, see HiSeq 2500 Requirements & Compatibility.
Contents and Storage
When you receive your kits, promptly store kit components at the indicated temperatures to ensure proper performance:
HiSeq Rapid Cluster Kit v2
HiSeq Rapid Cluster Kit v2 reagents are shipped in one box on dry ice. A two-lane rapid flow cell and gaskets are also included.
PE Cluster Kit, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
AMS |
Fast Amplification Mix |
1 |
FDR |
Fast Denaturation Reagent |
1 |
FLM1 |
Fast Linearization Mix 1 |
1 |
FLM2 |
Fast Linearization Mix 2 |
1 |
FPM |
Fast Premix |
1 |
FRM |
Fast Resynthesis Mix |
1 |
HP10 |
Read 1 Primer |
1 |
HP11 |
Read 2 Primer |
1 |
HP12 |
i7 Index Primer |
1 |
HT1 |
Hybridization Buffer |
SR Cluster Kit, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
AMS |
Fast Amplification Mix |
1 |
FDR |
Fast Denaturation Reagent |
1 |
FLS |
Fast Linearization Solution |
1 |
FPM |
Fast Premix |
1 |
HP9 |
i5 Index Primer |
1 |
HP10 |
Read 1 Primer |
1 |
HP12 |
i7 Index Primer |
1 |
HT1 |
Hybridization Buffer |
HiSeq Rapid SBS Kit v2
HiSeq Rapid SBS Kit v2 reagents are shipped in two boxes: one is shipped at room temperature and the other is shipped on dry ice. Reagents are sensitive to light. Store the reagent cartridge in a dark location away from light.
Reagents are labeled with a three-letter acronym plus a numeric designator that indicates the kit size. For example, if you are using CRM-50, the CRM was provided in a 50-cycle kit.
SBS Kit Box 1
Quantity |
Reagent |
Description |
Storage Temperature |
1 |
CWM |
Cleavage Wash Mix |
15°C to 30°C |
1 |
PW1 |
Wash Buffer |
-25°C to 30°C |
2 |
USB |
Universal Sequencing Buffer |
15°C to 30°C |
SBS Kit Box 2, Store at -25°C to -15°C
Quantity |
Reagent |
Description |
1 |
IMT |
Incorporation Master Mix |
1 |
CRM |
Cleavage Reagent Mix |
1 |
USM |
Universal Scan Mix |