NextSeq 550 Training

  • Introductory Topics

    DATE/LENGTH


  • NextSeq: Preparing for Your Install

    This course provides an overview of the key components of the NextSeq Series instruments, as well as the lab, computing, and electrical requirements for installation.

    15 min


  • NextSeq: System Overview

    By the end of this course, you will be able to: identify features of the NextSeq system, describe NextSeq sequencing technology, and describe the simplified sequencing workflow of the NextSeq system.

    20 min


  • How Do I Prepare for My Illumina Instrument Delivery?

    In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources. Note that, depending on your region, the steps shown in this video may not apply to the iSeq 100 system.

    7 min


  • Sequencing Analysis Viewer (SAV): A Beginner's Guide

    Recorded Webinar (May 2020) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.


  • Sequencing: Introduction to Sequencing By Synthesis (SBS)

    Recorded Webinar (June 2020) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.


  • Library Prep

    DATE/LENGTH


  • AmpliSeq for Illumina: Library Prep Protocol

    This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

    30 min


  • AmpliSeq for Illumina: Overview

    This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

    15 min


  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min


  • Nextera DNA Flex Library Preparation

    By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.

    20 min


  • Instruments & Software

    DATE/LENGTH


  • Discover the Benefits of Illumina Proactive

    Learn about the benefits of instrument performance monitoring through Illumina Proactive, a service in which instrument performance data are sent to Illumina to enable proactive maintenance.

    10 min


  • IT Requirements for Implementing Illumina Proactive

    This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.

    4 min


  • NextSeq: How to Start a Run

    By the end of this course, you will be able to: identify the steps to start a run on the NextSeq, describe how to prepare the reagent cartridge, and start a sequencing run using NCS.

    20 min


  • NextSeq: Does My Run Look Good?

    This course introduces four options for monitoring your NextSeq run. It also describes key metrics in NextSeq Control Software (NCS), Local Run Manager, Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.

    10 min


  • Local Run Manager v2

    This course shows you how to get started using the Local Run Manager v2 software to set up your sequencing runs and view analysis results.

    Languages: French, Italian, German, SpanishChinese (Simplified)

    20 min


  • Preparing Runs with BaseSpace Sequence Hub

    This course provides an overview of how to use the BaseSpace Sequence Hub Prep tab to enter sample information and parameters for your sequencing run.

    25 min


  • Data Analysis

    DATE/LENGTH


  • BaseSpace Sequence Hub: Introduction and Analysis Overview

    Recorded Webinar (May 2020) | llumina Technical Support invites you to learn about BaseSpace, Illumina’s cloud-based sequencing data analysis solution. In this presentation, we will discuss how to navigate the BaseSpace dashboard, general BaseSpace functionally, and tips and tricks for successful application and workflow utilization. We will cover the following topics: How to upload/view data in BaseSpace, discussion of major analysis workflows, general overview of how to analyze data on BaseSpace.

    Please note: BaseMount and BaseSpace CLI are alpha software releases; features and functionality may change over time, and support is directly through Illumina software development.


  • Diagnosing Suboptimal Clustering in Nonpatterned Flow Cells

    Learn which run metrics and thumbnail images to monitor during a run to diagnose over clustering of a nonpatterned flow cell.

    8 min


  • Optimal Cluster Density Best Practices

    This video discusses optimal cluster density and how over and under-clustering can affect your sequencing data. It also examines common clustering issues and ways to prevent them.

    10 min


  • Sequence Analysis Viewer (SAV): A Beginner's Guide (Spanish)

    Upcoming Webinar | El programa “Sequencing Analysis Viewer” (SAV) es una aplicación gratuita para ver importantes métricas de calidad generadas durante la secuenciación. Esta videoconferencia proporcionará una visita guiada para principiantes sobre cómo usar SAV, así como consejos para evaluar la información más pertinente al control de calidad. Esta presentación está dirigida a nuevos usuarios y cubrirá los siguientes temas: Cómo importar datos a SAV, Qué métricas están disponibles en cada pestaña del software, Comprender cuáles son las métricas más valiosas para la revisión de una corrida y dónde encontrarlas.

    Jul 22, 2020 PST