NextSeq 550 Documentation

System Documents

SIZE

DATE

NextSeq 500 and NextSeq 550 Sequencing System Guide

System overview and instructions for operating and maintaining the NextSeq 500 and NextSeq 550.

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NextSeq 500 and NextSeq 550 Sequencing System Guide

NextSeq 500 and NextSeq 550 Sequencing System Guide Translated into Japanese

5 MB

Sep 6, 2023

NextSeq 500 and NextSeq 550 Sequencing System Guide Translated into Korean

4 MB

Aug 29, 2023

Denature and Dilute Protocol Generator

Instructions for denaturing and diluting prepared libraries for sequencing.

Generate Protocol

Feb 28, 2025

NextSeq System Denature and Dilute Libraries Guide – Translations

Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the NextSeq 500 and 550 Systems.

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NextSeq 500 and NextSeq 550 Sequencing Systems Denature and Dilute Libraries Guide in Japanese

2 MB

Jan 13, 2022

NextSeq 500 and 550 Systems Denature and Dilute Libraries Guide in Korean

2 MB

Nov 15, 2021

NextSeq System Custom Primers Guide

Instructions for sequencing with custom primers on the NextSeq 500 and NextSeq 550 Systems.

Download

< 1 MB

Mar 8, 2020

NextSeq System Site Prep Guide

Lab specifications and requirements to prepare a site for the the NextSeq 500 and NextSeq 550 Systems.

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NextSeq 500 and NextSeq 550 System Site Prep Guide (15045113 v06)

< 1 MB

Jul 13, 2023

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Brazilian Portuguese

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Dutch

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in French

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in German

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Italian

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Japanese

1 MB

Aug 29, 2023

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Korean

< 1 MB

Jul 21, 2023

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Luxembourgish

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Russian

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Simplified Chinese

< 1 MB

May 28, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Site Prep Guide in Spanish

< 1 MB

May 28, 2020

NextSeq System Safety and Compliance Guides

Safety considerations, compliance markings, and regulatory statements for the NextSeq 500 and NextSeq 550 systems.

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NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide (15046564)

< 1 MB

Mar 8, 2020

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Brazilian Portuguese

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in French

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in German

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Italian

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Japanese

< 1 MB

Jun 8, 2016

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Korean

< 1 MB

Jul 14, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Luxembourgish

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Russian

< 1 MB

Feb 5, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Simplified Chinese

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Spanish

< 1 MB

Jan 7, 2021

NextSeq 500 and NextSeq 550 Sequencing Systems Safety and Compliance Guide in Ukrainian

< 1 MB

Jan 7, 2021

RFID Reader Compliance Guide (15041950 v01)

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Brazilian Portuguese

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Dutch

< 1 MB

Nov 22, 2019

RFID Reader Compliance Guide in French

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in German

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Italian

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Korean

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Luxembourgish

< 1 MB

Nov 22, 2019

RFID Reader Compliance Guide in Russian

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Simplified Chinese

2 MB

Nov 2, 2015

RFID Reader Compliance Guide in Spanish

2 MB

Nov 2, 2015

NextSeq 550 RUO Instrument v4.2.0 Software Release Notes

Download

< 1 MB

May 31, 2023

NextSeq System Suite Release Notes

Release notes for the NextSeq System Suite software.

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NextSeq 550 RUO v4.2.0 Software Customer Release Notes

< 1 MB

May 31, 2023

NextSeq v4.0.2 Software Release Notes (1000000132193 v00)

< 1 MB

Oct 1, 2020

NextSeq v4.0.1 Software Release Notes (1000000101366 v01)

< 1 MB

Sep 2, 2020

NextSeq v4.0.1 Software Release Notes Translated into Simplified Chinese

< 1 MB

Nov 4, 2019

NextSeq v4.0 Software Release Notes (1000000070338 v03)

< 1 MB

Sep 2, 2020

NextSeq RUO Software Suite Installer Release Notes (1000000051580 v00)

< 1 MB

Mar 13, 2018

NextSeq v2.2.0 Software Release Notes (1000000056025 v00)

< 1 MB

May 14, 2018

NextSeq v2.1.3 Software Release Notes (1000000017010 v01)

< 1 MB

Oct 24, 2017

NextSeq Software Release Notes, v2.0.2 (1000000004907-00)

< 1 MB

Nov 10, 2015

NextSeq System Software v2.0.1 Release Notes (15051678 K)

< 1 MB

Jun 24, 2015

NextSeq System Software v1.3.2 Release Notes (15071669 A)

< 1 MB

May 27, 2015

Product Literature	TYPE	SIZE	DATE
Laboratory Experience Using Illumina Next-Generation Sequencing for Noninvasive Prenatal Testing Application Note Download	Application note	< 1 MB	Mar 27, 2015
Illumina CMOS Chip and One-Channel SBS Chemistry Download	technology_spotlights	< 1 MB	Jan 16, 2014
NextSeq System Suite Installer Version 4.0 Upgrade Download	Technical note
Microbial whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation Download	Application note	< 1 MB	Jun 9, 2020
QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 System Download	Application note	2 MB	Jan 29, 2020
Direct Bacterial Colony Sequencing with the Nextera™ DNA Flex Library Preparation Kit Download	Application note	1 MB	Feb 21, 2018
Illumina Sequencing Platforms Brochure Download	Brochure	1 MB	Oct 9, 2024
NextSeq 550 System Specification Sheet Download	Data sheet	< 1 MB	Oct 28, 2022
Explore the transcriptome with single-cell resolution Download	Technical note	1 MB	Mar 1, 2022
Data Security with Illumina Proactive Download	Technical note	< 1 MB	Oct 4, 2022
Multiomic interrogation of the immune system at single-cell resolution Download	Technical note	1 MB	Mar 1, 2022
Unify single-cell gene expression and chromatin accessibility Download	Technical note	1 MB
Resolve the whole transcriptome within tissue architecture Download	Technical note	3 MB	Jul 1, 2021
Illumina DNA Prep crude lysate protocol for NGS Download	Application note	< 1 MB
Direct bacterial colony sequencing with Illumina DNA Prep Download	Application note	< 1 MB
NextSeq 550 amplicon sequencing solution Download	Application note	< 1 MB	Jan 29, 2021
NextSeq 550 exome sequencing solution Download	Application note	< 1 MB	Jan 29, 2021
NextSeq 550 RNA-Seq solution Download	Application note	< 1 MB	Oct 13, 2021

For every lab, everywhere

Illumina Single Cell 3' RNA Prep

NGS Workflow Finder

Multiomic data analysis for any lab

Illumina Proactive Instrument Performance Service

Do more, faster than ever

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

For every lab, everywhere

For every lab, everywhere

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For every lab, everywhere

NextSeq 550 Documentation

NextSeq 550 System

Documentation

System Documents

SIZE

DATE

NextSeq 500 and NextSeq 550 Sequencing System Guide

Denature and Dilute Protocol Generator

NextSeq System Denature and Dilute Libraries Guide – Translations

NextSeq System Custom Primers Guide

NextSeq System Site Prep Guide

NextSeq System Safety and Compliance Guides

NextSeq 550 RUO Instrument v4.2.0 Software Release Notes

NextSeq System Suite Release Notes

Product Literature

TYPE

SIZE